Zeiss KF 2 ICS Specifikace stáhnout pdf (Strana 69)

100

Operation

Axiostar plus Transmitted-light polarization Carl Zeiss

B 40-815 e 12/01 3-13

• If necessary, correct the orientation of the

analyzer to achieve an optimal, dark

viewing field.

• Move the object to be examined into the

field of view. Birefringent (anisotropic)

objects now usually exhibit the optical

effects described above.

☞

The orientation of the analyzer to the polarizer

changes when you turn the tube, which in turn

influences the contrast.

3.5.4 Sample differentiation between

gout and pseudogout

• Move two polarizers to the dark position

(analyzer NORTH-SOUTH-oriented, polarizer

EAST-WEST-oriented).

• Swivel in lambda plate and, if a rotary

lambda plate is available (e.g. 445226-

0000-000), set the oscillation direction to

45° (γ, stop position).

• Select crystal needles that are oriented in

the gamma direction (see marking on the

lambda plate).

Analysis:

• If the crystal needles oriented parallel to the

gamma direction of the lambda plate are

yellow, and the crystal needles lying at a

right angle to the gamma direction are

blue, the crystals are monosodium urate

crystals (gout).

• If the crystal needles oriented parallel to the

gamma direction of the lambda plate are

blue, and the crystal needles lying at a right

angle to the gamma direction are yellow,

the crystals are calcium pyrophosphate

crystals (pseudogout).

This analysis is also possible using a polarizer

with cemented lambda plate which can be

placed on the luminous-field diaphragm. In that

case, the lambda plate needs not be rotated.

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