Zeiss KF 2 ICS Specifikace stáhnout pdf (Strana 63)

100

Operation

Axiostar plus Transmitted-light phase contrast Carl Zeiss

B 40-815 e 12/01 3-7

3.3.3 Setting transmitted-light phase

contrast

Requirements:

– As described in chapter 2, the microscope is

ready for operation and switched on

according to section 3.1.

– The microscope must be set for

transmitted-light brightfield, as described in

section 3.2.

Settings:

• Swivel phase contrast objective, e.g.

40×/0.65 Ph 2 (3-5/10), into the beam path.

• Open luminous-field diaphragm (3-5/7) and

aperture diaphragm (3-5/9) or (3-6/3) on

the Abbe condenser with turret.

If a phase stop position (or darkfield position) is

set on the turret condenser, a green dot

appears in the window of the aperture

diaphragm indicating that the stop must be

opened completely (green dot on the aperture

diaphragm and in the window (3-6/4) are in the

same row.

• On the condenser, insert the slider for

phase contrast (3-5/8) with the same name

as on the phase contrast objective, e.g.

Ph 2, or set the relevant position on the

turret disk (3-6/2) of the Abbe condenser

with 5-position turret.

• Adjust the brightness.

• Check whether phase stop centering

complies with the figure (3-7). For this,

remove one eyepiece and replace it with the

diopter (3-5/11) or a auxiliary telescope

(3-5/3). Focus on the phase ring by pulling

out or pushing in the 3-5/1) of the auxiliary

telescope via the knurled ring (3-5/2).

• If required, center the phase stop (3-7/A)

via the two adjusting screws (3-5/6 or

(3-6/5) using a 90° hexagonal key SW 1.5.

• Then replace the diopter or the auxiliary

telescope with the eyepiece.

1 Turret disk

2 Window for turret disk position

3 Aperture diaphragm lever

4 Window for aperture diaphragm marking

5 Adjusting screws for phase stops

Fig. 3-6 Setting Abbe condenser with

5-position turret disk

Fig. 3-7 Centering phase stop

☞

Complete phase contrast is only achieved if the

bright phase stop (in the condenser) and the

dark phase ring (in the objective) are exactly

congruent in the illumination beam path

(3-7/B).

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