Zeiss KF 2 ICS Specifikace stáhnout pdf (Strana 60)

Operation

Carl Zeiss Transmitted-light brightfield Axiostar plus

3-4 B 40-81 e 12/01

3.2 Using the transmitted-light

brightfield technique

3.2.1 General principle

Transmitted-light brightfield microscopy is the

most usual of all the optical microscopy

techniques, since it allows the easy and fast

viewing of high-contrast or stained specimens

(e.g. blood smears).

In order to obtain optimum resolution with full

illumination of the field, the condenser, the

luminous-field diaphragm and the aperture

diaphragm must be set in accordance with the

rules of the KÖHLER illumination principle.

Here, the illumination cone is adapted to the

objective's opening cone. In this way, the

numerical aperture of the optical system is

used, and "superfluous" light, which can cause

interference in the form of scattered light, is

prevented.

1 Brightness control

2 Aperture diaphragm lever

3 Condenser drive

Fig. 3-3 Transmitted-light brightfield for KÖHLER

illumination, preparation

3.2.2 Transmitted-light brightfield

configuration

– Each Axiostar plus microscope can be

configured to permit the transmitted-light

brightfield technique.

– When using the Abbe condenser with 5-

position turret, set the brightfield position

(H) via the turret disk.

– If a slider for phase contrast is available with

the 0.9/1.25 Abbe condenser, it must be

removed when using the transmitted-light

brightfield technique.

3.2.3 Setting transmitted-light

brightfield for KÖHLER

illumination

Requirement:

– As described in section 2, the microscope is

ready for operation and switched on

according to section 3.1.

Settings:

• First, place a high-contrast specimen with

0.17 mm cover slip on top of the

mechanical stage 75x30 R/L.

• Set image brightness using the brightness

control (3-3/1) on the microscope stand.

• Move the Abbe condenser 0.9/1.25 to the

upper stop position via the condenser drive

(3-3/3) and move aperture diaphragm lever

(3-3/2) to the center position.

☞

If the microscope is equipped with a mechanical

stage 75 x 30 R (stage drive on the right side),

then the condenser drive will be attached on

the left in the factory.

The condenser drive will be located on the right

side of the microscope if the microscope is

equipped with a mechanical stage 75 x 30 L.

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