Zeiss Axiovert 200 Uživatelská příručka stáhnout pdf (Strana 2)

CONTENTS

1. General ................................................................................................................. 3

1.1 Instrument .................................................................................................................. 3

1.2 Reservations................................................................................................................ 3

1.3 Billing .......................................................................................................................... 3

1.4 Unauthorized use ........................................................................................................ 3

1.5 Users’ mailing list ........................................................................................................ 4

1.6 User guide ................................................................................................................... 4

1.7 Acknowledging MIU .................................................................................................... 4

1.8 Image processing and analysis ..................................................................................... 4

2. Microscope use ................................................................................................... 4

2.1 Settings for the light path ............................................................................................ 4

2.2 Objectives ................................................................................................................... 6

2.3 Fluorescence filters ..................................................................................................... 6

2.4 Transmitted (bright-field) light microscopy .................................................................. 7

2.4.1 Koehler illumination ............................................................................................. 7

2.4.2 Phase-contrast imaging ........................................................................................ 8

2.4.3 DIC imaging .......................................................................................................... 8

3. Imaging .............................................................................................................. 10

3.1 Zeiss AxioCam HRc 14-bit color CCD camera .............................................................. 10

3.2 Zeiss AxioVision software (version 3.1) ...................................................................... 10

3.2.1 Live view ............................................................................................................ 10

3.2.2 Adjustment of Camera Settings .......................................................................... 11

3.3 Display properties ..................................................................................................... 13

3.3.1 Adding a scale bar .............................................................................................. 14

4. Saving and transferring images ....................................................................... 15

4.1 File formats ............................................................................................................... 15

4.2 Back-up copies .......................................................................................................... 15

4.3 Saving images ............................................................................................................ 15

4.4 Saving images in Mcbserver1..................................................................................... 15

4.5 Saving images into your own computer ..................................................................... 16

4.6 Saving images into network folder............................................................................. 16

4.7 Saving images into a USB stick or CD ......................................................................... 16

4.8 Saving images locally ................................................................................................. 16

5. Ending your imaging session ........................................................................... 18

6. Troubleshooting ................................................................................................ 18

6.1 Microscope (both fluorescence and transmitted light imaging) ................................. 18

6.1.1 Fluorescence light microscopy ............................................................................ 18

6.1.2 Transmitted light microscopy ............................................................................. 19

6.2 Camera ..................................................................................................................... 19

6.4 AxioVision software ................................................................................................... 20

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Zeiss Axiovert 200 Uživatelská příručka Strana 2