Zeiss Axiovert 200 Uživatelský manuál stáhnout pdf (Strana 2)

27 Jan 2015,<qsg-wf1> p. 2/8

Startup procedure

Turn on (in this order):

• if required: heater unit and heater, 4 hours before live imaging to allow focus to stabilise:

•  plate holder

•  chamber

• if required: CO

supply

• switch on the mercury fluorescence lamp

• MAIN switch on the wall, on the right side of the microscope

• computer

• Log in with your local account (not your IC login) and start the Volocity software.

• Open or Create a Library

• If using stitching or multi-positions then calibrate stage

• Remove sample!!!

• Switch to shortest objective (10x, 2,5x or empty position)!!!

• Move objective turret to the lowest position!!!

• Choose "Calibrate Stage" from the "Stage" menu

Observing through the eyepiece

The microscope is best controlled through the software, although the filter cubes,

objectives etc can be selected manually.

Start the Volocity software and click on the camera icon and Select Objective

Select light path to “binoculars” using control buttons on the left-hand side of the

microscope (lowest button: - switches between Binocular (eyepiece) <> Baseport

(camera) <> Frontport (empty) <> Binocular (eyepiece)

Choose a pre-set button for your channel settings by clicking on one

- standard pre-sets for filter cubes are

 1 = Brightfield

 2 = DAPI/ Hoechst

 3 = GFP / FITC/ Alexa488

 4 = Rhodamine / TRITC/ Alexa543

 5+ = Other cubes on request

(mCherry, Texas Red, CY5

1 2 3 4 5 6 7 8

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Zeiss Axiovert 200 Uživatelský manuál Strana 2