Zeiss LSM 510 Uživatelský manuál stáhnout pdf (Strana 2)

Table of Contents

1. START HARDWARE....................................................................................................................... 3

2. START SOFTWARE........................................................................................................................ 4

3. START LASERS............................................................................................................................. 5

4. FIND THE SPECIMEN

(AXIOPLAN 2)

..................................................................................................... 6

4. FIND THE SPECIMEN

(AXIOVERT 200M)

................................................................................................. 7

5. CONFOCAL FILTER SET CONFIGURATION.................................................................................. 8

6. ACQUIRE PRELIMINARY CONFOCAL IMAGE............................................................................... 9

7. OPTIMIZING THE SETTINGS........................................................................................................ 10

7.1. GETTING THE DISPLAY READY.................................................................................................... 10

7.2. SCAN CONTROL: CHANNELS WINDOW......................................................................................... 12

7.3. ACQUIRING YOUR FINAL IMAGE .................................................................................................. 15

8. SAVING YOUR FINAL IMAGE ...................................................................................................... 17

9. ACQUIRING A Z-SERIES.............................................................................................................. 18

10. ADVANCED OPTIONS............................................................................................................. 20

10.1. SINGLE-TRACK – SIMULTANEOUS ACQUISITION ............................................................................ 20

10.1.1. Getting the display ready ............................................................................................. 20

10.1.2. Optimise settings......................................................................................................... 20

10.2. Z-ATTENUATION COMPENSATION................................................................................................ 21

10.3. TRANSMITTED LIGHT IMAGE....................................................................................................... 22

10.4. FRAP................................................................................................................................... 23

11. SHUTTING DOWN THE SYSTEM............................................................................................. 25

11.1. TURN OFF LASERS ................................................................................................................... 25

11.2. REMOVE SPECIMEN AND CLEAN MICROSCOPE .............................................................................. 25

11.3. EXIT THE SOFTWARE ................................................................................................................ 25

11.4. POWER DOWN THE SYSTEM ....................................................................................................... 25

12. OPENING YOUR IMAGES OFFLINE ........................................................................................ 26

12.1. ZEISS IMAGE BROWSER ............................................................................................................ 26

12.2. IMAGEJ ................................................................................................................................. 26

1 2 3 4 5 6 7 ... 25 26

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Zeiss LSM 510 Uživatelský manuál Strana 2