Zeiss LSM 510 META Uživatelský manuál stáhnout pdf (Strana 31)

Carl Zeiss - Training Application and Support Center

Zeiss LSM 510 META - Guided Tour

Minimal Pixel Size determined by Nyquist Sampling

Adjusting the field size (“XY”) to 56 µm with

the 63× lens, would produce a pixel size of

0.1 µm

Field size can be adjusted by changing the objective magnification, or by optical zooming. Changing

from 63 × to 100 × will reduce the field size, but will also reduce the amount of light available.

Brightness of image = Magnification

/NA

Values are for scan zoom = 1.0

100

0.15

0.3

0.5

1.3

(oil)

1.4 (

oil

)

1.4 (

oil

)

1.03 µm

0.51 µm

0.31 µm

0.12 µm

0.11 µm

Pixel sizeMagnification

1 2 ... 26 27 28 29 30 31 32 33 34 35 36 ... 65 66

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Zeiss LSM 510 META Uživatelský manuál Strana 31