Zeiss LSM 510 META Uživatelský manuál stáhnout pdf (Strana 6)

LSM 510 Meta Training Notes

The lasers

z Go to the Acquire tab and select Laser to open the Laser Control dialog that gives you access to the

three lasers on the Meta:

• Argon (30mW) 458,488, and 514 nm

• HeNe 1 (1.0 mW) 543 nm

• HeNe 1 (5.0 mW) 633 nm

z Select the laser(s) that would provide the correct wavelength(s) for exciting your specimen.

• Argon – This laser is fan cooled and has a specific start up and shut down procedure. Select

Standby, when Status indicates Ready, click On. Set Tube Current to ~6.1 -6.5 amps. This

should correspond to ~ 50% output.

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Zeiss LSM 510 META Uživatelský manuál Strana 6