Zeiss Axioplan Universal microscope Provozní pokyny Strana 12

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4 Instructions for use
4.3.3 Working in circularly polarized light.
The
Axiolab Pol
,
Axioplan 2 Pol
and
Axio-
phot 2 Pol
microscopes permit the universal ro-
tary stage to be used also for work in circularly
polarized light. For this, the circular polarizer D
consisting of a polarizer and two /4 plates
must be used. The polarizer and one of the /4
plates is used in the place of the standard po-
larizer. The second /4 plate is inserted in the
compensator mount. Before doing so, adjust
the polarizer and the analyzer so that they are
at a 90° angle to each other. Align the upper
/4 plate to the lower one until dark position is
reached without adjusting the objective. Cir-
cularly polarized light can be used in conjunc-
tion with the universal rotary stage to precisely
measure the angle of the optical axes 2 V in
birefringent, optically biaxial crystals, for exam-
ple. If you wish to work in linearly polarized light,
remove the two /4 plates from the beam
path.
Note:
For details on the use of circularly polar-
ized light, please refer to Zschach [15].
Example 3:Measuring the angle 2 V of the optical
axis of a biaxial crystal in circularly
polarized light
Proceed as described in
example 2
(steps 1 to
10).
Switch in the 50x/0.60 objective.
Switch in the Bertrand lens (focus
Axioplan
mi-
croscopes).
Use the graduated circle and the vernier
(Fig. 3/
19
) to measure the optical axis by tilting
about A
4.
The axial directions can be identified
by the two circular, dark zones with the exit
point of the optical axes in their centers .
Note:
If only one optical axis is located within
the tilting range about A
4
, proceed as
described in
example 1
.
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