
INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510 Functions
B 40-051 e 07/98 5-61
Method
The Input sequence defines the data to be reconstructed. If it is a multichannel sequence one or all
channels can be selected for the reconstruction.
Output sets the name of the result image (sequence). If the sequence exists it is overwritten. Pressing
the button New will generate a new name (number). The size of the sequential images in Output is
determined by the size of the sequential images in Input.
Number of Views determines the number of reconstructions which should be computed. The radio
buttons Start and End define which angle settings are currently shown. A definition for the angle End is
only necessary if Number of Views is higher than 1. If this is true the result sequence will get views
from the Start to the End angle definition. The other reconstructions are determined through the
linearly interpolated intermediate angles.
The direction of view is determined from the angles as follows:
The angle Angle Z determines the rotation of the direction of view on the Z-axis. The angle Angle Y
determines the rotation of the direction of view on the Y-axis that has been rotated by the angle
Angle Z. The angle Angle X determines the rotation of the direction of view on an X-axis that is rotated
by Angle Z and Angle Y.
Channel defines if the following parameters are valid for All or just for one. Defining the opacity for the
channels independently is useful when the brightness and contrast of the channels differ too much.
Threshold defines the range with no opacity. It is completely transparent. The range starts at grey
value 0.
The length of slope is defined by Ramp. The maximum opacity value is set with the parameter Max.
Opacity. This range ends at the maximum grey value. The Opacity Table shows the grey value
histogram of Input with the opacity definition as a red line.
When Auto Update is selected, the reconstruction is updated automatically whenever a parameter is
modified (except Input, Output, or Number of Views). Show Cube defines whether a wire frame
cube is shown in the Display window or not.
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